0	8	Distinct	distinct	JJ
9	14	roles	role	NNS
15	17	of	of	IN
18	21	the	the	DT
22	31	molecular	molecular	JJ
32	41	chaperone	chaperone	NN
42	47	hsp90	hsp90	NN
48	50	in	in	IN
51	61	modulating	modulate	VBG
62	68	dioxin	dioxin	NN
69	77	receptor	receptor	NN
78	86	function	function	NN
87	90	via	via	IN
91	94	the	the	DT
95	100	basic	basic	JJ
101	117	helix-loop-helix	helix-loop-helix	JJ
118	121	and	and	CC
122	125	PAS	PAS	NNP
126	133	domains	domain	NNS
133	134	.	.	.

136	139	The	the	DT
140	153	intracellular	intracellular	JJ
154	160	dioxin	dioxin	NN
161	169	receptor	receptor	NN
170	178	mediates	mediate	VBZ
179	185	signal	signal	NN
186	198	transduction	transduction	NN
199	201	by	by	IN
202	208	dioxin	dioxin	NN
209	212	and	and	CC
213	222	functions	function	VBZ
223	225	as	as	IN
226	227	a	a	DT
228	244	ligand-activated	ligand-activated	JJ
245	258	transcription	transcription	NN
259	265	factor	factor	NN
265	266	.	.	.

267	269	It	it	PRP
270	278	contains	contain	VBZ
279	280	a	a	DT
281	286	basic	basic	JJ
287	303	helix-loop-helix	helix-loop-helix	JJ
304	305	(	(	(
305	309	bHLH	bhlh	JJ
309	310	)	)	)
311	316	motif	motif	NN
317	327	contiguous	contiguous	JJ
328	332	with	with	IN
333	334	a	a	DT
335	347	Per-Arnt-Sim	Per-Arnt-Sim	NNP
348	349	(	(	(
349	352	PAS	PAS	NNP
352	353	)	)	)
354	362	homology	homology	NN
363	369	region	region	NN
369	370	.	.	.

371	373	In	in	IN
374	382	extracts	extract	NNS
383	387	from	from	IN
388	401	nonstimulated	nonstimulated	JJ
402	407	cells	cell	NNS
408	411	the	the	DT
412	420	receptor	receptor	NN
421	423	is	be	VBZ
424	433	recovered	recover	VBN
434	436	in	in	IN
437	439	an	an	DT
440	449	inducible	inducible	JJ
450	461	cytoplasmic	cytoplasmic	JJ
462	466	form	form	NN
467	477	associated	associate	VBN
478	482	with	with	IN
483	486	the	the	DT
487	493	90-kDa	90-kda	JJ
494	498	heat	heat	NN
499	504	shock	shock	NN
505	512	protein	protein	NN
513	514	(	(	(
514	519	hsp90	hsp90	NN
519	520	)	)	)
520	521	,	,	,
522	523	a	a	DT
524	533	molecular	molecular	JJ
534	543	chaperone	chaperone	NN
543	544	.	.	.

545	547	We	we	PRP
548	552	have	have	VBP
553	566	reconstituted	reconstitute	VBN
567	583	ligand-dependent	ligand-dependent	JJ
584	594	activation	activation	NN
595	597	of	of	IN
598	601	the	the	DT
602	610	receptor	receptor	NN
611	613	to	to	TO
614	615	a	a	DT
616	627	DNA-binding	dna-binding	JJ
628	632	form	form	NN
633	635	by	by	IN
636	641	using	use	VBG
642	645	the	the	DT
646	652	dioxin	dioxin	NN
653	661	receptor	receptor	NN
662	665	and	and	CC
666	669	its	its	PRP$
670	678	bHLH-PAS	bhlh-pas	JJ
679	686	partner	partner	NN
687	693	factor	factor	NN
694	698	Arnt	Arnt	NNP
699	708	expressed	express	VBN
709	711	by	by	IN
712	714	in	in	FW
715	720	vitro	vitro	FW
721	732	translation	translation	NN
733	735	in	in	IN
736	748	reticulocyte	reticulocyte	NN
749	755	lysate	lysate	NN
755	756	.	.	.

757	765	Deletion	deletion	NN
766	768	of	of	IN
769	772	the	the	DT
773	776	PAS	PAS	NNP
777	783	domain	domain	NN
784	786	of	of	IN
787	790	the	the	DT
791	799	receptor	receptor	NN
800	808	resulted	result	VBD
809	811	in	in	IN
812	824	constitutive	constitutive	JJ
825	837	dimerization	dimerization	NN
838	842	with	with	IN
843	847	Arnt	Arnt	NNP
847	848	.	.	.

849	851	In	in	IN
852	860	contrast	contrast	NN
860	861	,	,	,
862	866	this	this	DT
867	875	receptor	receptor	NN
876	882	mutant	mutant	NN
883	889	showed	show	VBD
890	893	low	low	JJ
894	900	levels	level	NNS
901	903	of	of	IN
904	914	xenobiotic	xenobiotic	JJ
915	923	response	response	NN
924	939	element-binding	element-binding	JJ
940	948	activity	activity	NN
948	949	,	,	,
950	960	indicating	indicate	VBG
961	965	that	that	IN
966	969	the	the	DT
970	973	PAS	PAS	NNP
974	980	domain	domain	NN
981	984	may	may	MD
985	987	be	be	VB
988	997	important	important	JJ
998	1001	for	for	IN
1002	1013	DNA-binding	dna-binding	JJ
1014	1022	affinity	affinity	NN
1023	1029	and/or	and/or	CC
1030	1041	specificity	specificity	NN
1042	1044	of	of	IN
1045	1048	the	the	DT
1049	1057	receptor	receptor	NN
1057	1058	.	.	.

1059	1061	It	it	PRP
1062	1065	was	be	VBD
1066	1069	not	not	RB
1070	1078	possible	possible	JJ
1079	1081	to	to	TO
1082	1094	reconstitute	reconstitute	VB
1095	1101	dioxin	dioxin	NN
1102	1110	receptor	receptor	NN
1111	1119	function	function	NN
1120	1124	with	with	IN
1125	1133	proteins	protein	NNS
1134	1143	expressed	express	VBN
1144	1146	in	in	IN
1147	1152	wheat	wheat	NN
1153	1157	germ	germ	NN
1158	1164	lysate	lysate	NN
1164	1165	.	.	.

1166	1168	In	in	IN
1169	1173	line	line	NN
1174	1178	with	with	IN
1179	1184	these	these	DT
1185	1197	observations	observation	NNS
1197	1198	,	,	,
1199	1211	reticulocyte	reticulocyte	NN
1212	1218	lysate	lysate	NN
1219	1222	but	but	CC
1223	1226	not	not	RB
1227	1232	wheat	wheat	NN
1233	1237	germ	germ	NN
1238	1244	lysate	lysate	NN
1245	1253	promoted	promote	VBD
1254	1257	the	the	DT
1258	1269	association	association	NN
1270	1272	of	of	IN
1273	1275	de	de	FW
1276	1280	novo	novo	FW
1281	1292	synthesized	synthesize	VBN
1293	1299	dioxin	dioxin	NN
1300	1308	receptor	receptor	NN
1309	1313	with	with	IN
1314	1319	hsp90	hsp90	NN
1319	1320	.	.	.

1321	1323	At	at	IN
1324	1329	least	least	JJS
1330	1333	two	two	CD
1334	1342	distinct	distinct	JJ
1343	1350	domains	domain	NNS
1351	1353	of	of	IN
1354	1357	the	the	DT
1358	1366	receptor	receptor	NN
1367	1375	mediated	mediated	JJ
1376	1387	interaction	interaction	NN
1388	1392	with	with	IN
1393	1398	hsp90	hsp90	NN
1398	1399	:	:	:
1400	1403	the	the	DT
1404	1418	ligand-binding	ligand-binding	JJ
1419	1425	domain	domain	NN
1426	1433	located	located	JJ
1434	1440	within	within	IN
1441	1444	the	the	DT
1445	1448	PAS	PAS	NNP
1449	1455	region	region	NN
1456	1459	and	and	CC
1459	1460	,	,	,
1461	1473	surprisingly	surprisingly	RB
1473	1474	,	,	,
1475	1478	the	the	DT
1479	1483	bHLH	bhlh	JJ
1484	1490	domain	domain	NN
1490	1491	.	.	.

1492	1499	Whereas	whereas	IN
1500	1514	ligand-binding	ligand-binding	JJ
1515	1523	activity	activity	NN
1524	1534	correlated	correlate	VBD
1535	1539	with	with	IN
1540	1551	association	association	NN
1552	1556	with	with	IN
1557	1562	hsp90	hsp90	NN
1562	1563	,	,	,
1564	1574	bHLH-hsp90	bhlh-hsp90	NN
1575	1586	interaction	interaction	NN
1587	1595	appeared	appear	VBD
1596	1598	to	to	TO
1599	1601	be	be	VB
1602	1611	important	important	JJ
1612	1615	for	for	IN
1616	1627	DNA-binding	dna-binding	JJ
1628	1636	activity	activity	NN
1637	1640	but	but	CC
1641	1644	not	not	RB
1645	1648	for	for	IN
1649	1661	dimerization	dimerization	NN
1662	1664	of	of	IN
1665	1668	the	the	DT
1669	1677	receptor	receptor	NN
1677	1678	.	.	.

1679	1686	Several	several	JJ
1687	1695	distinct	distinct	JJ
1696	1701	roles	role	NNS
1702	1705	for	for	IN
1706	1711	hsp90	hsp90	NN
1712	1714	in	in	IN
1715	1725	modulating	modulate	VBG
1726	1732	dioxin	dioxin	NN
1733	1741	receptor	receptor	NN
1742	1750	function	function	NN
1751	1754	are	be	VBP
1755	1764	therefore	therefore	RB
1765	1771	likely	likely	JJ
1771	1772	:	:	:
1773	1780	correct	correct	JJ
1781	1788	folding	folding	NN
1789	1791	of	of	IN
1792	1795	the	the	DT
1796	1810	ligand-binding	ligand-binding	JJ
1811	1817	domain	domain	NN
1817	1818	,	,	,
1819	1831	interference	interference	NN
1832	1836	with	with	IN
1837	1841	Arnt	Arnt	NNP
1842	1860	heterodimerization	heterodimerization	NN
1860	1861	,	,	,
1862	1865	and	and	CC
1866	1873	folding	folding	NN
1874	1876	of	of	IN
1877	1878	a	a	DT
1879	1890	DNA-binding	dna-binding	JJ
1891	1903	conformation	conformation	NN
1904	1906	of	of	IN
1907	1910	the	the	DT
1911	1915	bHLH	bhlh	JJ
1916	1922	domain	domain	NN
1922	1923	.	.	.

1924	1928	Thus	thus	RB
1928	1929	,	,	,
1930	1933	the	the	DT
1934	1940	dioxin	dioxin	NN
1941	1949	receptor	receptor	NN
1950	1956	system	system	NN
1957	1965	provides	provide	VBZ
1966	1967	a	a	DT
1968	1975	complex	complex	JJ
1976	1979	and	and	CC
1980	1991	interesting	interesting	JJ
1992	1997	model	model	NN
1998	2000	of	of	IN
2001	2004	the	the	DT
2005	2015	regulation	regulation	NN
2016	2018	of	of	IN
2019	2032	transcription	transcription	NN
2033	2040	factors	factor	NNS
2041	2043	by	by	IN
2044	2049	hsp90	hsp90	NN
2049	2050	.	.	.