0 1 A a DT 2 8 factor factor NN 9 13 that that WDT 14 23 regulates regulate VBZ 24 27 the the DT 28 33 class class NN 34 36 II ii CD 37 42 major major JJ 43 61 histocompatibility histocompatibility NN 62 69 complex complex NN 70 74 gene gene NN 75 78 DPA DPA NNP 79 81 is be VBZ 82 83 a a DT 84 90 member member NN 91 93 of of IN 94 95 a a DT 96 105 subfamily subfamily NN 106 108 of of IN 109 113 zinc zinc NN 114 120 finger finger NN 121 129 proteins protein NNS 130 134 that that WDT 135 143 includes include VBZ 144 145 a a DT 146 156 Drosophila drosophila FW 157 170 developmental developmental JJ 171 178 control control NN 179 186 protein protein NN 186 187 . . . 189 190 A a DT 191 196 novel novel JJ 197 200 DNA dna NN 201 209 sequence sequence NN 210 217 element element NN 218 224 termed term VBN 225 228 the the DT 229 230 J J NNP 231 238 element element NN 239 247 involved involve VBN 248 250 in in IN 251 254 the the DT 255 264 regulated regulate VBN 265 275 expression expression NN 276 278 of of IN 279 284 class class NN 285 287 II ii CD 288 293 major major JJ 294 312 histocompatibility histocompatibility NN 313 320 complex complex NN 321 326 genes gene NNS 327 330 was be VBD 331 339 recently recently RB 340 349 described describe VBN 349 350 . . . 351 353 To to TO 354 359 study study VB 360 364 this this DT 365 372 element element NN 373 376 and and CC 377 380 its its PRP$ 381 385 role role NN 386 388 in in IN 389 394 class class NN 395 397 II ii CD 398 402 gene gene NN 403 413 regulation regulation NN 414 421 further further RB 421 422 , , , 423 424 a a DT 425 429 cDNA cdna NN 430 437 library library NN 438 441 was be VBD 442 450 screened screen VBN 451 455 with with IN 456 471 oligonucleotide oligonucleotide NN 472 478 probes probe NNS 479 489 containing contain VBG 490 494 both both CC 495 498 the the DT 499 500 S S NNP 501 508 element element NN 509 512 and and CC 513 516 the the DT 517 523 nearby nearby JJ 524 525 J J NNP 526 533 element element NN 534 536 of of IN 537 540 the the DT 541 546 human human JJ 547 550 DPA DPA NNP 551 555 gene gene NN 555 556 . . . 557 564 Several several JJ 565 568 DNA dna NN 569 575 clones clone NNS 576 580 were be VBD 581 589 obtained obtain VBN 590 592 by by IN 593 597 this this DT 598 607 procedure procedure NN 607 608 , , , 609 612 one one CD 613 615 of of IN 616 621 which which WDT 621 622 , , , 623 628 clone clone NN 629 631 18 18 CD 631 632 , , , 633 635 is be VBZ 636 644 reported report VBN 645 648 and and CC 649 662 characterized characterize VBN 663 667 here here RB 667 668 . . . 669 671 It it PRP 672 679 encodes encode VBZ 680 681 a a DT 682 689 protein protein NN 690 699 predicted predict VBN 700 702 to to TO 703 710 contain contain VB 711 714 688 688 CD 715 720 amino amino JJ 721 725 acid acid NN 726 734 residues residue NNS 734 735 , , , 736 745 including include VBG 746 748 11 11 CD 749 753 zinc zinc NN 754 760 finger finger NN 761 767 motifs motif NNS 768 770 of of IN 771 774 the the DT 775 779 C2H2 c2h2 NN 780 784 type type NN 785 787 in in IN 788 791 the the DT 792 802 C-terminal c-terminal JJ 803 809 region region NN 809 810 , , , 811 815 that that WDT 816 819 are be VBP 820 832 Kruppel-like kruppel-like JJ 833 835 in in IN 836 839 the the DT 840 852 conservation conservation NN 853 855 of of IN 856 859 the the DT 860 863 H/C H/C NNP 864 868 link link NN 869 877 sequence sequence NN 878 888 connecting connect VBG 889 893 them them PRP 893 894 . . . 895 898 The the DT 899 902 160 160 CD 903 913 N-terminal n-terminal JJ 914 919 amino amino NN 920 925 acids acid NNS 926 928 in in IN 929 932 the the DT 933 942 nonfinger nonfinger JJ 943 949 region region NN 950 952 of of IN 953 958 clone clone NN 959 961 18 18 CD 962 965 are be VBP 966 972 highly highly RB 973 983 homologous homologous JJ 984 988 with with IN 989 996 similar similar JJ 997 1004 regions region NNS 1005 1007 of of IN 1008 1015 several several JJ 1016 1021 other other JJ 1022 1027 human human JJ 1027 1028 , , , 1029 1034 mouse mouse NN 1034 1035 , , , 1036 1039 and and CC 1040 1050 Drosophila drosophila FW 1051 1060 sequences sequence NNS 1060 1061 , , , 1062 1070 defining define VBG 1071 1072 a a DT 1073 1082 subfamily subfamily NN 1083 1085 of of IN 1086 1098 Kruppel-like kruppel-like JJ 1099 1103 zinc zinc NN 1104 1110 finger finger NN 1111 1119 proteins protein NNS 1120 1126 termed term VBN 1127 1130 TAB TAB NNP 1131 1132 ( ( ( 1132 1141 tramtrack tramtrack NN 1142 1143 [ [ ( 1143 1158 ttk]-associated ttk]-associated JJ 1159 1162 box box NN 1162 1163 ) ) ) 1164 1168 here here RB 1168 1169 . . . 1170 1173 One one CD 1174 1176 of of IN 1177 1180 the the DT 1181 1191 Drosophila drosophila FW 1192 1201 sequences sequence NNS 1201 1202 , , , 1203 1206 ttk ttk NN 1206 1207 , , , 1208 1210 is be VBZ 1211 1212 a a DT 1213 1226 developmental developmental JJ 1227 1234 control control NN 1235 1239 gene gene NN 1239 1240 , , , 1241 1246 while while IN 1247 1248 a a DT 1249 1255 second second JJ 1256 1260 does do VBZ 1261 1264 not not RB 1265 1272 contain contain VB 1273 1274 a a DT 1275 1279 zinc zinc NN 1280 1286 finger finger NN 1287 1293 region region NN 1294 1297 but but CC 1298 1305 encodes encode VBZ 1306 1307 a a DT 1308 1317 structure structure NN 1318 1327 important important JJ 1328 1330 in in IN 1331 1337 oocyte oocyte NN 1338 1349 development development NN 1349 1350 . . . 1351 1353 An an DT 1354 1360 acidic acidic JJ 1361 1371 activation activation NN 1372 1378 domain domain NN 1379 1381 is be VBZ 1382 1389 located located JJ 1390 1397 between between IN 1398 1401 the the DT 1402 1412 N-terminal n-terminal JJ 1413 1422 conserved conserve VBN 1423 1429 region region NN 1430 1432 of of IN 1433 1438 clone clone NN 1439 1441 18 18 CD 1442 1445 and and CC 1446 1449 its its PRP$ 1450 1454 zinc zinc NN 1455 1462 fingers finger NNS 1462 1463 . . . 1464 1468 This this DT 1469 1476 protein protein NN 1477 1484 appears appear VBZ 1485 1487 to to TO 1488 1495 require require VB 1496 1500 both both CC 1501 1504 the the DT 1505 1506 S S NNP 1507 1510 and and CC 1511 1512 J J NNP 1513 1521 elements element NNS 1521 1522 , , , 1523 1528 which which WDT 1529 1532 are be VBP 1533 1542 separated separate VBN 1543 1545 by by IN 1546 1548 10 10 CD 1549 1551 bp bp NN 1552 1555 for for IN 1556 1563 optimal optimal JJ 1564 1571 binding binding NN 1571 1572 . . . 1573 1582 Antisense antisense JJ 1583 1587 cDNA cDNA NNP 1588 1590 to to TO 1591 1596 clone clone NN 1597 1599 18 18 CD 1600 1609 inhibited inhibit VBD 1610 1613 the the DT 1614 1624 expression expression NN 1625 1627 of of IN 1628 1629 a a DT 1630 1638 reporter reporter NN 1639 1648 construct construct NN 1649 1659 containing contain VBG 1660 1663 the the DT 1664 1667 DPA DPA NNP 1668 1676 promoter promoter NN 1676 1677 , , , 1678 1688 indicating indicate VBG 1689 1692 its its PRP$ 1693 1703 functional functional JJ 1704 1714 importance importance NN 1715 1717 in in IN 1718 1721 the the DT 1722 1732 expression expression NN 1733 1735 of of IN 1736 1740 this this DT 1741 1746 class class NN 1747 1749 II ii CD 1750 1754 gene gene NN 1754 1755 . . .