0 12 Differential differential JJ 13 22 induction induction NN 23 25 of of IN 26 37 DNA-binding dna-binding JJ 38 48 activities activity NNS 49 58 following follow VBG 59 63 CD19 cd19 NN 64 77 cross-linking cross-linking NN 78 80 in in IN 81 86 human human JJ 87 88 B B NNP 89 96 lineage lineage NN 97 102 cells cell NNS 102 103 . . . 105 108 The the DT 109 110 B B NNP 111 124 cell-specific cell-specific JJ 125 129 cell cell NN 130 137 surface surface NN 138 146 molecule molecule NN 147 151 CD19 cd19 NN 152 154 is be VBZ 155 164 expressed express VBN 165 167 at at IN 168 171 all all DT 172 178 stages stage NNS 179 181 of of IN 182 183 B B NNP 184 188 cell cell NN 189 200 development development NN 200 201 , , , 202 211 including include VBG 212 218 normal normal JJ 219 225 plasma plasma NN 226 231 cells cell NNS 231 232 , , , 233 236 and and CC 237 245 mediates mediate VBZ 246 252 signal signal NN 253 265 transduction transduction NN 266 269 via via IN 270 281 interaction interaction NN 282 286 with with IN 287 298 cytoplasmic cytoplasmic JJ 299 307 effector effector NN 308 316 proteins protein NNS 316 317 . . . 318 331 Cross-linking cross-linking JJ 332 336 CD19 cd19 NN 337 339 on on IN 340 345 early early JJ 346 351 human human JJ 352 353 B B NNP 354 361 lineage lineage NN 362 367 cells cell NNS 368 375 induces induce VBZ 376 379 the the DT 380 389 formation formation NN 390 392 of of IN 393 394 a a DT 395 426 CD19/Vav/phosphatidylinositol-3 CD19/Vav/phosphatidylinositol-3 NNP 427 433 kinase kinase NNP 434 441 complex complex NN 441 442 , , , 443 451 tyrosine tyrosine NN 452 467 phosphorylation phosphorylation NN 468 470 of of IN 471 475 CD19 CD19 NNP 476 479 and and CC 480 483 Vav Vav NNP 483 484 , , , 485 488 and and CC 489 499 activation activation NN 500 502 of of IN 503 506 the the DT 507 510 Ras Ras NNP 511 518 pathway pathway NN 518 519 . . . 520 522 To to TO 523 530 further further RB 531 538 explore explore VB 539 542 the the DT 543 556 ramifications ramification NNS 557 559 of of IN 560 564 CD19 cd19 NN 565 574 signaling signaling NN 574 575 , , , 576 579 the the DT 580 587 current current JJ 588 593 study study NN 594 602 examined examine VBD 603 610 whether whether IN 611 626 phosphorylation phosphorylation NN 627 629 of of IN 630 635 Elk-1 Elk-1 NNP 635 636 , , , 637 647 activation activation NN 648 650 of of IN 651 660 activator activator NN 661 670 protein-1 protein-1 NN 671 672 ( ( ( 672 676 AP-1 ap-1 NN 676 677 ) ) ) 677 678 , , , 679 681 or or CC 682 692 activation activation NN 693 695 of of IN 696 703 nuclear nuclear JJ 704 717 factor-kappaB factor-kappaB NNP 718 719 ( ( ( 719 728 NF-kappaB NF-kappaB NNP 728 729 ) ) ) 730 743 transcription transcription NN 744 751 factors factor NNS 752 760 occurred occur VBD 761 770 following follow VBG 771 775 CD19 cd19 NN 776 789 cross-linking cross-linking NN 789 790 . . . 791 794 The the DT 795 800 cells cell NNS 801 805 used use VBN 806 810 were be VBD 811 814 the the DT 815 821 BLIN-1 blin-1 NN 822 827 pre-B pre-b JJ 828 832 cell cell NN 833 837 line line NN 838 848 expressing express VBG 849 852 low low JJ 853 859 levels level NNS 860 862 of of IN 863 867 cell cell NN 868 875 surface surface NN 876 878 mu mu NN 879 884 heavy heavy JJ 885 890 chain chain NN 891 901 associated associate VBN 902 906 with with IN 907 916 surrogate surrogate JJ 917 922 light light JJ 923 928 chain chain NN 929 932 and and CC 933 936 the the DT 937 940 1E8 1e8 NN 941 949 immature immature JJ 950 951 B B NNP 952 956 cell cell NN 957 961 line line NN 962 972 expressing express VBG 973 977 cell cell NN 978 985 surface surface NN 986 994 mu/kappa mu/kappa NN 994 995 . . . 996 1003 Lysates lysate NNS 1004 1008 from from IN 1009 1013 CD19 cd19 NN 1014 1026 cross-linked cross-linked JJ 1027 1030 1E8 1e8 NN 1031 1036 cells cell NNS 1037 1044 induced induce VBD 1045 1051 robust robust JJ 1052 1067 phosphorylation phosphorylation NN 1068 1070 of of IN 1071 1073 an an DT 1074 1079 Elk-1 elk-1 NN 1080 1086 fusion fusion NN 1087 1094 protein protein NN 1095 1097 in in FW 1098 1103 vitro vitro FW 1103 1104 , , , 1105 1112 whereas whereas IN 1113 1115 no no DT 1116 1131 phosphorylation phosphorylation NN 1132 1134 of of IN 1135 1140 Elk-1 elk-1 NN 1141 1147 fusion fusion NN 1148 1155 protein protein NN 1156 1164 occurred occur VBD 1165 1170 using use VBG 1171 1178 lysates lysate NNS 1179 1183 from from IN 1184 1188 CD19 cd19 NN 1189 1201 cross-linked cross-linked JJ 1202 1208 BLIN-1 blin-1 NN 1209 1214 cells cell NNS 1214 1215 . . . 1216 1218 An an DT 1219 1234 electrophoretic electrophoretic JJ 1235 1243 mobility mobility NN 1244 1249 shift shift NN 1250 1255 assay assay NN 1256 1265 employing employ VBG 1266 1270 AP-1 ap-1 NN 1271 1274 and and CC 1275 1284 NF-kappaB NF-kappaB NNP 1285 1294 consensus consensus NN 1295 1311 oligonucleotides oligonucleotide NNS 1312 1315 was be VBD 1316 1320 used use VBN 1321 1323 to to TO 1324 1335 demonstrate demonstrate VB 1336 1340 that that IN 1341 1345 AP-1 ap-1 NN 1346 1354 -binding -binding JJ 1355 1363 activity activity NN 1364 1373 increased increase VBD 1373 1374 , , , 1375 1380 while while IN 1381 1393 constitutive constitutive JJ 1394 1411 NF-kappaB-binding nf-kappab-binding JJ 1412 1420 activity activity NN 1421 1424 was be VBD 1425 1428 not not RB 1429 1437 enhanced enhance VBN 1437 1438 , , , 1439 1448 following follow VBG 1449 1450 2 2 CD 1451 1452 h h NN 1453 1455 of of IN 1456 1460 CD19 cd19 NN 1461 1474 cross-linking cross-linking NN 1475 1477 in in IN 1478 1481 1E8 1e8 NN 1482 1487 cells cell NNS 1487 1488 . . . 1489 1499 Supershift Supershift NNP 1500 1511 experiments experiment NNS 1512 1520 revealed reveal VBD 1521 1525 that that IN 1526 1530 JunD JunD NNP 1531 1534 and and CC 1535 1540 c-Fos c-fos NN 1541 1549 proteins protein NNS 1550 1558 mediated mediate VBN 1559 1568 anti-CD19 anti-cd19 NN 1569 1576 induced induce VBD 1577 1589 AP-1-binding ap-1-binding JJ 1590 1598 activity activity NN 1599 1601 in in IN 1602 1605 1E8 1e8 NN 1606 1611 cells cell NNS 1611 1612 . . . 1613 1615 In in IN 1616 1624 contrast contrast NN 1624 1625 , , , 1626 1630 CD19 cd19 NN 1631 1644 cross-linking cross-linking NN 1645 1647 in in IN 1648 1654 BLIN-1 blin-1 NN 1655 1660 cells cell NNS 1661 1669 resulted result VBD 1670 1672 in in IN 1673 1676 the the DT 1677 1686 induction induction NN 1687 1689 of of IN 1690 1699 NF-kappaB NF-kappaB NNP 1699 1700 , , , 1701 1704 but but CC 1705 1708 had have VBD 1709 1711 no no DT 1712 1720 apparent apparent JJ 1721 1727 effect effect NN 1728 1730 on on IN 1731 1743 AP-1-binding ap-1-binding JJ 1744 1752 activity activity NN 1752 1753 . . . 1754 1759 These these DT 1760 1764 data datum NNS 1765 1772 suggest suggest VBP 1773 1777 that that IN 1778 1791 CD19-mediated cd19-mediated JJ 1792 1798 signal signal NN 1799 1811 transduction transduction NN 1812 1821 activates activate VBZ 1822 1831 different different JJ 1832 1845 transcription transcription NN 1846 1853 factors factor NNS 1854 1856 at at IN 1857 1867 juxtaposed juxtapose VBN 1868 1874 stages stage NNS 1875 1877 of of IN 1878 1879 B B NNP 1880 1884 cell cell NN 1885 1896 development development NN 1897 1901 that that WDT 1902 1905 may may MD 1906 1915 culminate culminate VB 1916 1918 in in IN 1919 1922 the the DT 1923 1933 activation activation NN 1934 1936 or or CC 1937 1948 suppression suppression NN 1949 1951 of of IN 1952 1960 distinct distinct JJ 1961 1965 sets set NNS 1966 1968 of of IN 1969 1974 genes gene NNS 1974 1975 . . .